Protein variants form a system of networks: microdiversity of IMP metallo-beta-lactamases.

ABSTRACT: Genome and metagenome sequencing projects support the view that only a tiny portion of the total protein microdiversity in the biosphere has been sequenced yet, while the vast majority of existing protein variants is still unknown. By using a network approach, the microdiversity of 42 metallo-β-lactamases of the IMP family was investigated. In the networks, the nodes are formed by the variants, while the edges correspond to single mutations between pairs of variants. The 42 variants were assigned to 7 separate networks. By analyzing the networks and their relationships, the structure of sequence space was studied and existing, but still unknown, functional variants were predicted. The largest network consists of 10 variants with IMP-1 in its center and includes two ubiquitous mutations, V6

SUBMITTER: Widmann M 

PROVIDER: S-EPMC4094381 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature