Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer.

ABSTRACT:

Introduction

Serum profiling using proteomic techniques has great potential to detect biomarkers that might improve diagnosis and predict outcome for breast cancer patients (BC). This study used surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) to identify differentially expressed proteins in sera from BC and healthy volunteers (HV), with the goal of developing a new prognostic biomarker panel.

Methods

Training set serum samples from 99 BC and 51 HV subjects were applied to four adsorptive chip surfaces (anion-exchange, cation-exchange, hydrophobic, and metal affinity) and analyzed by time-of-flight MS. For validation, 100 independent BC serum samples and 70 HV samples were analyzed similarly. Cluster analysis of protein spectra w

SUBMITTER: Chung L 

PROVIDER: S-EPMC4095593 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature