Decreased interleukin 27 expression is associated with active uveitis in Behcet's disease.

ABSTRACT:

Instruction

Interleukin 27 (IL-27) is an important regulator of the proinflammatory T-cell response. In this study, we investigated its role in the pathogenesis of Behçet's disease (BD).

Methods

IL-27 mRNA in peripheral blood mononuclear cells (PBMCs) was examined by performing RT-PCRs. Cytokine levels in sera or supernatants of PBMCs, naïve CD4(+) T cells, dendritic cells (DCs) and DC/T cells were determined by enzyme-linked immunosorbent assay. We used RNA interference in naïve CD4(+) T cells to study the role of interferon regulatory factor 8 (IRF8) in the inhibitory effect of IL-27 on Th17 cell differentiation. Flow cytometry was used to evaluate the frequency of IL-17- and interferon γ-producing T cells.

Results

The expression of IL-27p28 mRNA by PBMCs and IL-27

SUBMITTER: Wang C 

PROVIDER: S-EPMC4095700 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature