Integrated analysis identifies different metabolic signatures for tumor-initiating cells in a murine glioblastoma model.

ABSTRACT:

Background

The metabolic preference of malignant glioma for glycolysis as an energy source is a potential therapeutic target. As a result of the cellular heterogeneity of these tumors, however, the relation between glycolytic preference, tumor formation, and tumor cell clonogenicity has remained unknown. To address this issue, we analyzed the metabolic profiles of isogenic glioma-initiating cells (GICs) in a mouse model.

Methods

GICs were established by overexpression of H-Ras(V12) in Ink4a/Arf-null neural stem cells. Subpopulations of these cells were obtained by single-cell cloning, and clones differing in extracellular acidification potential were assessed for metabolic characteristics. Tumors formed after intracranial implantation of these clones in mice were examined fo

SUBMITTER: Saga I 

PROVIDER: S-EPMC4096179 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature