Bioinformatic and proteomic analysis of bulk histones reveals PTM crosstalk and chromatin features.

ABSTRACT: Systems analysis of chromatin has been constrained by complex patterns and dynamics of histone post-translational modifications (PTMs), which represent major challenges for both mass spectrometry (MS) and immuno-based approaches (e.g., chromatin immuno-precipitation, ChIP). Here we present a proof-of-concept study demonstrating that crosstalk among PTMs and their functional significance can be revealed via systematic bioinformatic and proteomic analysis of steady-state histone PTM levels from cells under various perturbations. Using high resolution tandem MS, we quantified 53 modification states from all core histones and their conserved variants in the unicellular eukaryotic model organism Tetrahymena. By correlating histone PTM patterns across 15 different conditions, including various p

SUBMITTER: Zhang C 

PROVIDER: S-EPMC4096215 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature