Hydrogel-based 3D model of patient-derived prostate xenograft tumors suitable for drug screening.

ABSTRACT: The lack of effective therapies for bone metastatic prostate cancer (PCa) underscores the need for accurate models of the disease to enable the discovery of new therapeutic targets and to test drug sensitivities of individual tumors. To this end, the patient-derived xenograft (PDX) PCa model using immunocompromised mice was established to model the disease with greater fidelity than is possible with currently employed cell lines grown on tissue culture plastic. However, poorly adherent PDX tumor cells exhibit low viability in standard culture, making it difficult to manipulate these cells for subsequent controlled mechanistic studies. To overcome this challenge, we encapsulated PDX tumor cells within a three-dimensional hyaluronan-based hydrogel and demonstrated that the hydrogel maintains

SUBMITTER: Fong EL 

PROVIDER: S-EPMC4096229 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature