Role of global and local topology in the regulation of gene expression in Streptococcus pneumoniae.

ABSTRACT: The most basic level of transcription regulation in Streptococcus pneumoniae is the organization of its chromosome in topological domains. In response to drugs that caused DNA-relaxation, a global transcriptional response was observed. Several chromosomal domains were identified based on the transcriptional response of their genes: up-regulated (U), down-regulated (D), non-regulated (N), and flanking (F). We show that these distinct domains have different expression and conservation characteristics. Microarray fluorescence units under non-relaxation conditions were used as a measure of gene transcriptional level. Fluorescence units were significantly lower in F genes than in the other domains with a similar AT content. The transcriptional level of the domains categorized them was D>U>F. In

SUBMITTER: Ferrandiz MJ 

PROVIDER: S-EPMC4096756 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature