Zygote serine decreased uptake from the fertilization medium is associated with implantation and pregnancy.

ABSTRACT:

Purpose

To identify an association between amino acids (AAs) metabolism and reproductive outcome.

Methods

Prospective collection, observational study, in patients undergoing fresh, double embryo transfer (ET), in a tertiary hospital referral IVF unit. Spent day 1 and day 3 media were collected. Concentrations of taurine, aspartic acid, proline, and serine in the medium were determined using a liquid-chromatography mass-spectrometer (LCMS/MS). Data was analyzed according to excretion versus uptake, and a cut-off value was calculated based on a receiver operating curve (ROC). Pregnancy rates were also calculated after stratification into subgroups in accordance with AA metabolism.

Results

Seven out of 19 patients conceived (36.8 %). The ORs for pregnancy when the zygote

SUBMITTER: Zivi E 

PROVIDER: S-EPMC4096884 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature