Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro.

ABSTRACT:

Purpose

The levels and timing of expression of genes like BCLXL, HDAC1 and pluripotency marker genes namely, OCT4, SOX2, NANOG and KLF4 are known to influence preimplantation embryo development. Despite this information, precise understanding of their influence during preimplantation embryo development is lacking. The present study attempts to compare the expression of these genes in the in vivo and in vitro developed preimplantation embryos.

Methods

The in vivo and in vitro developed rabbit embryos collected at distinct developmental stages namely, pronuclear, 2 cell, 4 cell, 8 cell, 16 cell, Morula and blastocyst were compared at the transcriptional and translational levels using Real Time PCR and immunocytochemical studies respectively.

Results

The study establishe

SUBMITTER: Henderson GR 

PROVIDER: S-EPMC4096891 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature