Proteins of the ciliary axoneme are found on cytoplasmic membrane vesicles during growth of cilia.

ABSTRACT: The cilium is a specialized extension of the cell in which many specific proteins are admitted and retained, while many others are excluded or expelled. In order to maintain the organelle, the cell must possess mechanisms for the selective gating of protein entry, as well as for the targeted transport of proteins to the cilium from their sites of synthesis within the cell [1-4]. We hypothesized that the cell employs cytoplasmic vesicles as vehicles not only for the transport of proteins destined for the ciliary membrane but also for the transport of axonemal proteins to the cilium by means of peripheral association with vesicles. To test this hypothesis, we employed two different experimental strategies: (1) isolation and biochemical characterization of cytoplasmic vesicles that carry cili

SUBMITTER: Wood CR 

PROVIDER: S-EPMC4096978 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature