DNA adsorption to and elution from silica surfaces: influence of amino acid buffers.

ABSTRACT: Solid phase extraction and purification of DNA from complex samples typically requires chaotropic salts that can inhibit downstream polymerase amplification if carried into the elution buffer. Amino acid buffers may serve as a more compatible alternative for modulating the interaction between DNA and silica surfaces. We characterized DNA binding to silica surfaces, facilitated by representative amino acid buffers, and the subsequent elution of DNA from the silica surfaces. Through bulk depletion experiments, we found that more DNA adsorbs to silica particles out of positively compared to negatively charged amino acid buffers. Additionally, the type of the silica surface greatly influences the amount of DNA adsorbed and the final elution yield. Quartz crystal microbalance experiments with d

SUBMITTER: Vandeventer PE 

PROVIDER: S-EPMC4097040 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature