Silencing Met receptor tyrosine kinase signaling decreased oral tumor growth and increased survival of nude mice.

ABSTRACT:

Objectives

The hepatocyte growth factor receptor (Met) is frequently overexpressed in Head and Neck Squamous Cell Carcinoma (HNSCC), correlating positively with high-grade tumors and shortened patient survival. As such, Met may represent an important therapeutic target. The purpose of this study was to explore the role of Met signaling for HNSCC growth and locoregional dissemination.

Materials and methods

Using a lentiviral system for RNA interference, we knocked down Met in established HNSCC cell lines that express high levels of the endogenous receptor. The effect of Met silencing on in vitro proliferation, cell survival and migration was examined using western analysis, immunohistochemistry and live cell imaging. In vivo tumor growth, dissemination and mouse survival was

SUBMITTER: Tao X 

PROVIDER: S-EPMC4097605 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature