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Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification.

ABSTRACT: Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.

SUBMITTER: Curtis KA 

PROVIDER: S-EPMC4097747 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification.

Curtis Kelly A KA   Niedzwiedz Philip L PL   Youngpairoj Ae S AS   Rudolph Donna L DL   Owen S Michele SM  

Journal of clinical microbiology 20140430 7

Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device. ...[more]

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