Diabetes impairs the angiogenic potential of adipose-derived stem cells by selectively depleting cellular subpopulations.

ABSTRACT:

Introduction

Pathophysiologic changes associated with diabetes impair new blood vessel formation and wound healing. Mesenchymal stem cells derived from adipose tissue (ASCs) have been used clinically to promote healing, although it remains unclear whether diabetes impairs their functional and therapeutic capacity.

Methods

In this study, we examined the impact of diabetes on the murine ASC niche as well as on the potential of isolated cells to promote neovascularization in vitro and in vivo. A novel single-cell analytical approach was used to interrogate ASC heterogeneity and subpopulation dynamics in this pathologic setting.

Results

Our results demonstrate that diabetes alters the ASC niche in situ and that diabetic ASCs are compromised in their ability to establish a

SUBMITTER: Rennert RC 

PROVIDER: S-EPMC4097831 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature