Novel principles of gamma-retroviral insertional transcription activation in murine leukemia virus-induced end-stage tumors.

ABSTRACT:

Background

Insertional mutagenesis screens of retrovirus-induced mouse tumors have proven valuable in human cancer research and for understanding adverse effects of retroviral-based gene therapies. In previous studies, the assignment of mouse genes to individual retroviral integration sites has been based on close proximity and expression patterns of annotated genes at target positions in the genome. We here employed next-generation RNA sequencing to map retroviral-mouse chimeric junctions genome-wide, and to identify local patterns of transcription activation in T-lymphomas induced by the murine leukemia gamma-retrovirus SL3-3. Moreover, to determine epigenetic integration preferences underlying long-range gene activation by retroviruses, the colocalization propensity with common

SUBMITTER: Sokol M 

PROVIDER: S-EPMC4098794 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature