Comparative secretome analysis of Streptomyces scabiei during growth in the presence or absence of potato suberin.

ABSTRACT:

Background

Suberin is a recalcitrant plant biopolymer composed of a polyphenolic and a polyaliphatic domain. Although suberin contributes to a significant portion of soil organic matter, the biological process of suberin degradation is poorly characterized. It has been suggested that Streptomyces scabiei, a plant pathogenic bacterium, can produce suberin-degrading enzymes. In this study, a comparative analysis of the S. scabiei secretome from culture media supplemented or not with potato suberin was carried out to identify enzymes that could be involved in suberin degradation.

Methods

S. scabiei was grown in the presence of casein only or in the presence of both casein and suberin. Extracellular proteins from 1-, 3- and 5-day-old supernatants were analyzed by LC-MS/MS to det

SUBMITTER: Komeil D 

PROVIDER: S-EPMC4098958 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature