Characterization of the nasopharyngeal microbiota in health and during rhinovirus challenge.

ABSTRACT:

Background

The bacterial communities of the nasopharynx play an important role in upper respiratory tract infections (URTIs). Our study represents the first survey of the nasopharynx during a known, controlled viral challenge. We aimed to gain a better understanding of the composition and dynamics of the nasopharyngeal microbiome during viral infection.

Methods

Rhinovirus illnesses were induced by self-inoculation using the finger to nose or eye natural transmission route in ten otherwise healthy young adults. Nasal lavage fluid samples (NLF) samples were collected at specific time points before, during, and following experimental rhinovirus inoculation. Bacterial DNA from each sample (N = 97 from 10 subjects) was subjected to 16S rRNA sequencing by amplifying the V1-V2 hype

SUBMITTER: Allen EK 

PROVIDER: S-EPMC4098959 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature