Single-stranded DNA catalyzes hybridization of PCR-products to microarray capture probes.

ABSTRACT: Since its development, microarray technology has evolved to a standard method in the biotechnological and medical field with a broad range of applications. Nevertheless, the underlying mechanism of the hybridization process of PCR-products to microarray capture probes is still not completely understood, and several observed phenomena cannot be explained with current models. We investigated the influence of several parameters on the hybridization reaction and identified ssDNA to play a major role in the process. An increase of the ssDNA content in a hybridization reaction strongly enhanced resulting signal intensities. A strong influence could also be observed when unlabeled ssDNA was added to the hybridization reaction. A reduction of the ssDNA content resulted in a massive decrease of the

SUBMITTER: Dally S 

PROVIDER: S-EPMC4099319 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature