Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases.

ABSTRACT:

Background

The past decade has seen the emergence of several molecular tools that render possible modification of cellular functions through accurate and easy addition, removal, or exchange of genomic DNA sequences. Among these technologies, transcription activator-like effectors (TALE) has turned out to be one of the most versatile and incredibly robust platform for generating targeted molecular tools as demonstrated by fusion to various domains such as transcription activator, repressor and nucleases.

Results

In this study, we generated a novel nuclease architecture based on the transcription activator-like effector scaffold. In contrast to the existing Tail to Tail (TtT) and head to Head (HtH) nuclease architectures based on the symmetrical association of two TALE DNA bin

SUBMITTER: Juillerat A 

PROVIDER: S-EPMC4099384 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature