A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome.

ABSTRACT:

Background

Multiple tick saliva proteins, the majority of which are unknown, confer tick resistance in repeatedly infested animals. The objective of this study was to identify the 24-48 h fed Amblyomma americanum tick saliva immuno-proteome. The 24-48 h tick-feeding phase is critical to tick parasitism as it precedes important events in tick biology, blood meal feeding and disease agent transmission. Fed male, 24 and 96 h fed female phage display cDNA expression libraries were biopanned using rabbit antibodies to 24 and 48 h fed A. americanum female tick saliva proteins. Biopanned immuno-cDNA libraries were subjected to next generation sequencing, de novo assembly, and bioinformatic analysis.

Results

More than 800 transcripts that code for 24-48 h fed A. americanum immuno-pr

SUBMITTER: Radulovic ZM 

PROVIDER: S-EPMC4099483 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature