Persistence of Bacteroides ovatus under simulated sunlight irradiation.

ABSTRACT:

Background

Bacteroides ovatus, a member of the genus Bacteroides, is considered for use in molecular-based methods as a general fecal indicator. However, knowledge on its fate and persistence after a fecal contamination event remains limited. In this study, the persistence of B. ovatus was evaluated under simulated sunlight exposure and in conditions similar to freshwater and seawater. By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence and absence of exogenous photosensitizers and in salinity up to 39.5 parts per thousand at 27°C.

Results

UVB was found to be important for B. ovatus decay, averaging a 4 log10 of decay over 6 h of exposure without the presenc

SUBMITTER: Dong S 

PROVIDER: S-EPMC4099502 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature