A simple three-step method for design and affinity testing of new antisense peptides: an example of erythropoietin.

ABSTRACT: Antisense peptide technology is a valuable tool for deriving new biologically active molecules and performing peptide-receptor modulation. It is based on the fact that peptides specified by the complementary (antisense) nucleotide sequences often bind to each other with a higher specificity and efficacy. We tested the validity of this concept on the example of human erythropoietin, a well-characterized and pharmacologically relevant hematopoietic growth factor. The purpose of the work was to present and test simple and efficient three-step procedure for the design of an antisense peptide targeting receptor-binding site of human erythropoietin. Firstly, we selected the carboxyl-terminal receptor binding region of the molecule (epitope) as a template for the antisense peptide modeling; Secon

SUBMITTER: Stambuk N 

PROVIDER: S-EPMC4100090 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature