Proteome analysis of subsarcolemmal cardiomyocyte mitochondria: a comparison of different analytical platforms.

ABSTRACT: Mitochondria are complex organelles that play critical roles in diverse aspects of cellular function. Heart disease and a number of other pathologies are associated with perturbations in the molecular machinery of the mitochondria. Therefore, comprehensive, unbiased examination of the mitochondrial proteome represents a powerful approach toward system-level insights into disease mechanisms. A crucial aspect in proteomics studies is design of bioanalytical strategies that maximize coverage of the complex repertoire of mitochondrial proteins. In this study, we evaluated the performance of gel-based and gel-free multidimensional platforms for profiling of the proteome in subsarcolemmal mitochondria harvested from rat heart. We compared three different multidimensional proteome fractionation p

SUBMITTER: Giorgianni F 

PROVIDER: S-EPMC4100094 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature