A cautionary note on the use of split-YFP/BiFC in plant protein-protein interaction studies.

ABSTRACT: Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescence after the two non-fluorescent halves of a fluorescent protein are brought together by a protein-protein interaction event. The major drawback of BiFC is that the fluorescent protein halves are prone to self-assembly independent of a protein-protein interaction event. To circumvent this problem, several modifications of the technique have been suggested, but these modifications have not lead to improvements in plant BiFC protocols. Therefore, it remains crucial to include appropriate internal

SUBMITTER: Horstman A 

PROVIDER: S-EPMC4100113 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature