A new criterion to evaluate water vapor interference in protein secondary structural analysis by FTIR spectroscopy.

ABSTRACT: Second derivative and Fourier self-deconvolution (FSD) are two commonly used techniques to resolve the overlapped component peaks from the often featureless amide I band in Fourier transform infrared (FTIR) curve-fitting approach for protein secondary structural analysis. Yet, the reliability of these two techniques is greatly affected by the omnipresent water vapor in the atmosphere. Several criteria are currently in use as quality controls to ensure the protein absorption spectrum is negligibly affected by water vapor interference. In this study, through a second derivative study of liquid water, we first argue that the previously established criteria cannot guarantee a reliable evaluation of water vapor interference due to a phenomenon that we refer to as sample's absorbance-dependent w

SUBMITTER: Zou Y 

PROVIDER: S-EPMC4100137 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature