Highly sensitive amplicon-based transcript quantification by semiconductor sequencing.

ABSTRACT:

Background

In clinical and basic research custom panels for transcript profiling are gaining importance because only project specific informative genes are interrogated. This approach reduces costs and complexity of data analysis and allows multiplexing of samples. Polymerase-chain-reaction (PCR) based TaqMan assays have high sensitivity but suffer from a limited dynamic range and sample throughput. Hence, there is a gap for a technology able to measure expression of large gene sets in multiple samples.

Results

We have adapted a commercially available mRNA quantification assay (AmpliSeq-RNA) that measures mRNA abundance based on the frequency of PCR amplicons determined by high-throughput semiconductor sequencing. This approach allows for parallel, accurate quantification of

SUBMITTER: Zhang JD 

PROVIDER: S-EPMC4101174 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature