Imaging mass spectrometry in papillary thyroid carcinoma for the identification and validation of biomarker proteins.

ABSTRACT: Direct tissue imaging mass spectrometry (IMS) by matrix-assisted laser desorption ionization and time-of-flight (MALDI-TOF) mass spectrometry has become increasingly important in biology and medicine, because this technology can detect the relative abundance and spatial distribution of interesting proteins in tissues. Five thyroid cancer samples, along with normal tissue, were sliced and transferred onto conductive glass slides. After laser scanning by MALDI-TOF equipped with a smart beam laser, images were created for individual masses and proteins were classified at 200-µm spatial resolution. Based on the spatial distribution, region-specific proteins on a tumor lesion could be identified by protein extraction from tumor tissue and analysis using liquid chromatography with tandem mass sp

SUBMITTER: Min KW 

PROVIDER: S-EPMC4101781 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature