A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram.

ABSTRACT: Myosin interacting-heads (MIH) motifs are visualized in 3D-reconstructions of thick filaments from striated muscle. These reconstructions are calculated by averaging methods using images from electron micrographs of grids prepared using numerous filament preparations. Here we propose an alternative method to calculate the 3D-reconstruction of a single thick filament using only a tilt series images recorded by electron tomography. Relaxed thick filaments, prepared from tarantula leg muscle homogenates, were negatively stained. Single-axis tilt series of single isolated thick filaments were obtained with the electron microscope at a low electron dose, and recorded on a CCD camera by electron tomography. An IHRSR 3D-recontruction was calculated from the tilt series images of a single thick fi

SUBMITTER: Marquez G 

PROVIDER: S-EPMC4102135 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature