Fit-for-purpose curated database application in mass spectrometry-based targeted protein identification and validation.

ABSTRACT:

Background

Mass spectrometry (MS) is a very sensitive and specific method for protein identification, biomarker discovery, and biomarker validation. Protein identification is commonly carried out by comparing MS data with public databases. However, with the development of high throughput and accurate genomic sequencing technology, public databases are being overwhelmed with new entries from different species every day. The application of these databases can also be problematic due to factors such as size, specificity, and unharmonized annotation of the molecules of interest. Current databases representing liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based searches focus on enzyme digestion patterns and sequence information and consequently, important functional informa

SUBMITTER: Cheng K 

PROVIDER: S-EPMC4102332 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature