RNA expression analysis of passive transfer myasthenia supports extraocular muscle as a unique immunological environment.

ABSTRACT:

Purpose

Myasthenia gravis demonstrates a distinct predilection for involvement of the extraocular muscles (EOM), and we have hypothesized that this may be due to a unique immunological environment. To assess this hypothesis, we took an unbiased approach to analyze RNA expression profiles in EOM, diaphragm, and extensor digitorum longus (EDL) in rats with experimentally acquired myasthenia gravis (EAMG).

Methods

Experimentally acquired myasthenia gravis was induced in rats by intraperitoneal injection of antibody directed against the acetylcholine receptor (AChR), whereas control rats received antibody known to bind the AChR but not induce disease. After 48 hours, animals were killed and muscles analyzed by RNA expression profiling. Profiling results were validated using qPCR

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC4102389 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature