Stability curve prediction of homologous proteins using temperature-dependent statistical potentials.

ABSTRACT: The unraveling and control of protein stability at different temperatures is a fundamental problem in biophysics that is substantially far from being quantitatively and accurately solved, as it requires a precise knowledge of the temperature dependence of amino acid interactions. In this paper we attempt to gain insight into the thermal stability of proteins by designing a tool to predict the full stability curve as a function of the temperature for a set of 45 proteins belonging to 11 homologous families, given their sequence and structure, as well as the melting temperature (Tm) and the change in heat capacity (ΔCP) of proteins belonging to the same family. Stability curves constitute a fundamental instrument to analyze in detail the thermal stability and its relation to the thermodynami

SUBMITTER: Pucci F 

PROVIDER: S-EPMC4102405 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature