Transcriptome sequencing and developmental regulation of gene expression in Anopheles aquasalis.

ABSTRACT:

Background

Anopheles aquasalis is a major malaria vector in coastal areas of South and Central America where it breeds preferentially in brackish water. This species is very susceptible to Plasmodium vivax and it has been already incriminated as responsible vector in malaria outbreaks. There has been no high-throughput investigation into the sequencing of An. aquasalis genes, transcripts and proteins despite its epidemiological relevance. Here we describe the sequencing, assembly and annotation of the An. aquasalis transcriptome.

Methodology/principal findings

A total of 419 thousand cDNA sequence reads, encompassing 164 million nucleotides, were assembled in 7544 contigs of ≥ 2 sequences, and 1999 singletons. The majority of the An. aquasalis transcripts encode proteins wit

SUBMITTER: Costa-da-Silva AL 

PROVIDER: S-EPMC4102416 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature