Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cells.

ABSTRACT:

Background

Elevated level of reactive carbonyl species, such as methylglyoxal, triggers carbonyl stress and activates a series of inflammatory responses leading to accelerated vascular damage. Edaravone is the active substance of a Japanese medicine, which aids neurological recovery following acute brain ischemia and subsequent cerebral infarction. Our aim was to test whether edaravone can exert a protective effect on the barrier properties of human brain endothelial cells (hCMEC/D3 cell line) treated with methylglyoxal.

Methodology

Cell viability was monitored in real-time by impedance-based cell electronic sensing. The barrier function of the monolayer was characterized by measurement of resistance and flux of permeability markers, and visualized by immunohistochemistry fo

SUBMITTER: Toth AE 

PROVIDER: S-EPMC4102474 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature