Metabolomic analysis of pressure-overloaded and infarcted mouse hearts.

ABSTRACT:

Background

Cardiac hypertrophy and heart failure are associated with metabolic dysregulation and a state of chronic energy deficiency. Although several disparate changes in individual metabolic pathways have been described, there has been no global assessment of metabolomic changes in hypertrophic and failing hearts in vivo. Hence, we investigated the impact of pressure overload and infarction on myocardial metabolism.

Methods and results

Male C57BL/6J mice were subjected to transverse aortic constriction or permanent coronary occlusion (myocardial infarction [MI]). A combination of LC/MS/MS and GC/MS techniques was used to measure 288 metabolites in these hearts. Both transverse aortic constriction and MI were associated with profound changes in myocardial metabolism affect

SUBMITTER: Sansbury BE 

PROVIDER: S-EPMC4102656 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature