Proteogenomic mapping of Mycoplasma hyopneumoniae virulent strain 232.

ABSTRACT:

Background

Mycoplasma hyopneumoniae causes respiratory disease in swine and contributes to the porcine respiratory disease complex, a major disease problem in the swine industry. The M. hyopneumoniae strain 232 genome is one of the smallest and best annotated microbial genomes, containing only 728 annotated genes and 691 known proteins. Standard protein databases for mass spectrometry only allow for the identification of known and predicted proteins, which if incorrect can limit our understanding of the biological processes at work. Proteogenomic mapping is a methodology which allows the entire 6-frame genome translation of an organism to be used as a mass spectrometry database to help identify unknown proteins as well as correct and confirm existing annotations. This methodology w

SUBMITTER: Pendarvis K 

PROVIDER: S-EPMC4102725 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature