Prognostic significance of CD44 variant 2 upregulation in colorectal cancer.

ABSTRACT:

Background

CD133 and CD44 are putative cancer stem cell (CSC) markers in colorectal cancer (CRC). However, their clinical significance is currently unclear. Here, we evaluated primary CRC cell isolates to determine the significance of several CSC markers, including CD133 and CD44, as predictors of tumourigenesis and prognosis.

Methods

CD133- and CD44-positive cells from fresh clinical samples of 77 CRCs were selected by flow cytometric sorting and evaluated for tumourigenicity following subcutaneous transplantation into NOD/SCID mice. Cancer stem cell marker expression was examined in both xenografts and a complementary DNA library compiled from 167 CRC patient samples.

Results

CD44(+), CD133(+) and CD133(+)CD44(+) sub-populations were significantly more tumourigenic

SUBMITTER: Ozawa M 

PROVIDER: S-EPMC4102936 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature