Dichloroacetate induces autophagy in colorectal cancer cells and tumours.

ABSTRACT:

Background

Dichloroacetate (DCA) has been found to have antitumour properties.

Methods

We investigated the cellular and metabolic responses to DCA treatment and recovery in human colorectal (HT29, HCT116 WT and HCT116 Bax-ko), prostate carcinoma cells (PC3) and HT29 xenografts by flow cytometry, western blotting, electron microscopy, (1)H and hyperpolarised (13)C-magnetic resonance spectroscopy.

Results

Increased expression of the autophagy markers LC3B II was observed following DCA treatment both in vitro and in vivo. We observed increased production of reactive oxygen species (ROS) and mTOR inhibition (decreased pS6 ribosomal protein and p4E-BP1 expression) as well as increased expression of MCT1 following DCA treatment. Steady-state lactate excretion and the appare

SUBMITTER: Lin G 

PROVIDER: S-EPMC4102941 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature