Multiplexed microfluidic enzyme assays for simultaneous detection of lipolysis products from adipocytes.

ABSTRACT: Microfluidics has enabled new cell biology experiments. Incorporating chemical monitoring of cellular secretion into chips offers the potential to increase information content and utility of such systems. In this work, an integrated, multilayer polydimethylsiloxane microfluidic chip was developed to simultaneously measure fatty acids and glycerol secreted from cultured adipocytes on chip in near real time. Approximately 48,000 adipocytes were loaded into a cell chamber in a reversibly sealed chip. Cells were perfused at 0.75 μL/min. Cell perfusate was split and directed to separate, continuously operating fluorescent enzyme assay channel networks. The fluorescent assay products were detected simultaneously near the outlet of the chip. The fatty acid and glycerol assays had linear dynamic r

SUBMITTER: Dugan CE 

PROVIDER: S-EPMC4103022 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature