Isolation of 18 microsatellite loci in the desert mistletoe Phoradendron californicum (Santalaceae) via 454 pyrosequencing.

ABSTRACT: Microsatellite primers were developed for the parasitic mistletoe Phoradendron californicum to investigate to what extent population genetic structure depends on host tree distribution within a highly fragmented landscape. • Fourteen unlinked polymorphic and four monomorphic nuclear microsatellite markers were developed using a genomic shotgun pyrosequencing method. A total of 187 alleles plus four monomorphic loci alleles were found in 98 individuals sampled in three populations from the Sonoran Desert in the Baja California peninsula (Mexico). Loci averaged 13.3 alleles per locus (range 4-28), and observed and expected heterozygosities within populations varied from 0.167-0.879 and 0.364-0.932, respectively. • Levels of polymorphism of the reported markers are adequate for studies of div

SUBMITTER: Arroyo JM 

PROVIDER: S-EPMC4103116 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature