Rapid staining method to detect and identify downy mildew (Peronospora belbahrii) in basil.

ABSTRACT:

Unlabelled

Premise of the study

Demand for fresh-market sweet basil continues to increase, but in 2009 a new pathogen emerged, threatening commercial field/greenhouse production and leading to high crop losses. This study describes a simple and effective staining method for rapid microscopic detection of basil downy mildew (Peronospora belbahrii) from leaves of basil (Ocimum basilicum). •

Methods and results

Fresh leaf sections infected with P. belbahrii were placed on a microscope slide, cleared with Visikol™, and stained with iodine solution followed by one drop of 70% sulfuric acid. Cell walls of the pathogen were stained with a distinct coloration, providing a high-contrast image between the pathogen and plant. •

Conclusions

This new staining method can be

SUBMITTER: Koroch AR 

PROVIDER: S-EPMC4103132 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature