Determination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).

ABSTRACT: Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The resu

SUBMITTER: Lecrenier MC 

PROVIDER: S-EPMC4103152 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature