EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees.

ABSTRACT: Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We used activation tagging to isolate a dominant mutation affecting release from dormancy and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and retransformation experiments that EBB1 encodes a putative APETALA2/Ethylene responsive factor transcription factor. Transgenic up-regulation of the gene caused early bud-flush, whereas down-regulation delayed bud-break. Native EBB1 expression was highest in actively growing apices, undetectable during the dormancy period, but rapidly increased before bud-break. The EBB1 transcript was localized in the L1/L2 layers of the shoot meristem

SUBMITTER: Yordanov YS 

PROVIDER: S-EPMC4103365 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature