Characterization of microsatellites in Xanthosoma sagittifolium (Araceae) and cross-amplification in related species.

ABSTRACT: To investigate the genetic diversity of a root crop, Xanthosoma sagittifolium, and to facilitate germplasm conservation, microsatellite loci were developed and characterized by genotyping 39 accessions from different geographic origins. • Using a microsatellite-enriched library approach, 17 polymorphic microsatellite markers were identified and characterized. The number of alleles for each locus ranged from two to six. Observed and expected heterozygosities ranged from 0.00 to 0.97 and from 0.09 to 0.78, respectively. Additionally, cross-amplification of these microsatellite markers was tested successfully in other species of Xanthosoma and Caladium, with rates varying from 23.5% to 100%. • These results indicate the effectiveness of microsatellite loci developed for the characterization o

SUBMITTER: Cathebras C 

PROVIDER: S-EPMC4103442 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature