Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae).

ABSTRACT:

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Premise of the study

Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. •

Methods and results

Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. •

Conclusions

The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.

SUBMITTER: Jain S 

PROVIDER: S-EPMC4103456 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature