Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae).

ABSTRACT: This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2-11), expected heterozygosity (0.226-0.883), and polymorphism information content per locus (0.212-0.870). Cross-amplification in C. argentea was successful in 16 loci, 12 of which were polymorphic (2-10 alleles). • The polymorphism of this set of microsatellite markers for C. mollis, as well as their successful cross-amplification in C. intermedia and C.

SUBMITTER: Lopez-Roberts MC 

PROVIDER: S-EPMC4103464 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature