Microsatellite markers for the New Zealand endemic tree Fuchsia excorticata (Onagraceae).

ABSTRACT: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymorphic within at least three populations and consistently amplified in the four populations tested. These primers amplified di-, tri-, and tetranucleotide repeats with 1-10 alleles per population. • These results indicate the utility of microsatellite loci for future mating system and population genetic studies in F. excorticata.

SUBMITTER: Van Etten ML 

PROVIDER: S-EPMC4103472 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature