Characterization of microsatellite loci in lichen-forming fungi of Bryoria section Implexae (Parmeliaceae).

ABSTRACT: The locally rare, haploid, lichen-forming fungi Bryoria capillaris, B. fuscescens, and B. implexa are associated with boreal forests and belong to Bryoria sect. Implexae. Recent phylogenetic studies consider them to be conspecific. Microsatellite loci were developed to study population structure in Bryoria sect. Implexae and its response to ecosystem disturbances. • We developed 18 polymorphic microsatellite markers using 454 pyrosequencing data assessed in 82 individuals. The number of alleles per locus ranged from two to 13 with an average of 4.6. Nei's unbiased gene diversity, averaged over loci, ranged from 0.38 to 0.52. The markers amplified with all three species, except for markers Bi05, Bi15, and Bi18. • The new markers will allow the study of population subdivision, levels of gene

SUBMITTER: Nadyeina O 

PROVIDER: S-EPMC4103479 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature