A target enrichment method for gathering phylogenetic information from hundreds of loci: An example from the Compositae.

ABSTRACT:

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Premise of the study

The Compositae (Asteraceae) are a large and diverse family of plants, and the most comprehensive phylogeny to date is a meta-tree based on 10 chloroplast loci that has several major unresolved nodes. We describe the development of an approach that enables the rapid sequencing of large numbers of orthologous nuclear loci to facilitate efficient phylogenomic analyses. •

Methods and results

We designed a set of sequence capture probes that target conserved orthologous sequences in the Compositae. We also developed a bioinformatic and phylogenetic workflow for processing and analyzing the resulting data. Application of our approach to 15 species from across the Compositae resulted in the production of phylogenetically informative sequence

SUBMITTER: Mandel JR 

PROVIDER: S-EPMC4103609 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature