Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease.

ABSTRACT:

Purpose

Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD.

Methods

The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis.

Results

Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immuno

SUBMITTER: Chase JM 

PROVIDER: S-EPMC4103907 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature