High-throughput phenotyping of chlamydomonas swimming mutants based on nanoscale video analysis.

ABSTRACT: Studies on biflagellated algae Chlamydomonas reinhardtii mutants have resulted in significant contributions to our understanding of the functions of cilia/flagella components. However, visual inspection conducted under a microscope to screen and classify Chlamydomonas swimming requires considerable time, effort, and experience. In addition, it is likely that identification of mutants by this screening is biased toward individual cells with severe swimming defects, and mutants that swim slightly more slowly than wild-type cells may be missed by these screening methods. To systematically screen Chlamydomonas swimming mutants, we have here developed the cell-locating-with-nanoscale-accuracy (CLONA) method to identify the cell position to within 10-nm precision through the analysis of high-spe

SUBMITTER: Fujita S 

PROVIDER: S-EPMC4104059 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature